Composite

Part:BBa_K310008

Designed by: Erin Borchardt   Group: iGEM10_UIUC-Illinois   (2010-09-30)

B0034+ ArsR + B0015

ArsR, a transcriptional repressor of the Ars Operon hooked up with the RBS B0034 and the terminator B0015 for expression when placed under the control of a promoter.

This paper was particularly helpful when working with the Ars Operon and Arsenic parts within E. coli: [http://aem.asm.org/cgi/reprint/70/8/4582 Enhanced Arsenic Accumulation in Engineered Bacterial Cells Expressing ArsR]

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 156


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